Plant-pollinator interactions are threatened by human activities. In the Tibetan Plateau, grazing is one of the main anthropic disturbances, and overgrazing has led to degradation of alpine meadow. However, it is still unclear how grazing affects plant-pollinator interaction network of alpine meadows. Here we collected data from two locations in the eastern Tibetan Plateau to evaluate the effects of grazing on the pollination networks in alpine meadow. Each location comprised one ungrazed plot (fenced during growing season) and one grazed plot (grazed all year). We conducted surveys during growing seasons (July and August) in three consecutive years (2016-2018), and constructed 16 pollination networks in total. The plants-pollinators interactions were surveyed using timed observation method by cameras. We found that the pollinator communities of alpine meadow were dominated by Diptera species. The richness of Diptera, Lepidoptera and Coleoptera pollinators were decreased after grazing, while the richness of Hymenoptera pollinators was unaffected by grazing. Grazing reduced the diversity of plants, pollinators and their interactions. The generalism of plants was also decreased by grazing, but the nestedness and specialization (H₂') of networks were similar between treatments, indicated that the stability and resilience of pollination networks are unaffected by grazing. Furthermore, the network metrics were similar between different months, and grazing had similar impacts on pollination networks in July and August. Our study firstly investigated the effect of the grazing on the pollination networks of alpine meadows in the Tibetan Plateau. The results highlighted that the grazing intensity of study area is exceeded the optimum intensity as it decreased the diversity of pollinators and pollination networks. Further studies should focus on how pollination networks response to grazing intensity in alpine meadow to find a reasonable grazing regime.