The sustainable use of ecosystem services can contribute to enhancing human well-being. Understanding the effects of land use on ecosystem services can provide scientific and targeted guidance for the sustainable use of ecosystem services. The characteristics of land use change were systematically analyzed by using the single land use dynamic degree on the basis of land use change in Hotan area from 2010 to 2018. Subsequently, the equivalent coefficient was modified and willingness to pay and ability to pay were introduced in the evaluation model, which was reconstructed to measure its ecosystem services value. The results showed that (1) Land use changed were more significant in Hotan area from 2010 to 2018. The cultivated land, urban villages, industrial and mining land and transportation land showed an increasing trend, whereas garden land, forest land, grassland, water area, and unused land showed a decreasing trend. (2) During 2010-2018, the total ecosystem service value in the study area has been decreasing, and reduction amounts were about 5.89×10⁸ yuan. The ecosystem service values of land use types were all had a decreasing trend, in which water area had the largest contribution. The regional ecological function adjustment service value was larger than that in support services and in cultural service and in supply service, in which hydrological regulation and waste treatment was the most important single service function. (3) On the spatial scale, the total ecosystem service value for Hotan County significantly decreased. The ecosystem service value for Minfen County was the highest,and that of Hotan city was the lowest. (4) The sensitivity indexes of ecosystem service value coefficient of all land use types were less than 1, indicating that the ecosystem service value was inelastic to the value coefficient, and the results were credible. The above results can not only provide basic data for inter-regional ecological compensation, but also serve as reference cases for other regions to carry out relevant research work.