The integration and coordinated development of Beijing, Tianjin and Hebei region (BTHr) is a national strategy. The primary tasks of this strategy are to expand ecological space and environmental capacity, and strengthen the cross-regional cooperation of ecological environment protection in the BTHr. Clarifying the distribution of biodiversity in the BTHr is the premise and foundation for the implementation of ecological environmental protection projects and for the allocation of national parks. We assessed the biodiversity conservation value of nature reserves in the BTHr. The assessment used the forestry industry standard "Technical Regulation for Assessing Biodiversity Conservation Value of Nature Reserves (LY/T 2649-2016)" and was based on scientific investigations of nature reserves in the BTHr, In addition, we constructed a multivariate regression model in which the biodiversity conservation value of the nature reserves was the dependent variable and the comprehensive landforms of the nature reserves were the independent variables. Based on the average area of the nature reserves, we divided the BTHr into 1638 grid cells using the "fishing net" function of ArcGIS. The biodiversity conservation value of each grid cell was evaluated by using the multivariate regression model. The average conservation value of the national nature reserves in the BTHr was 204 points, which was 40 points higher than the average conservation value of the total 35 nature reserves. Some provincial nature reserves, such as Tanghai wetland nature reserve and Nandagang nature reserve, also had comparatively high conservation values, which exceeded the average conservation value of the national nature reserves. Biodiversity conservation priority areas were selected according to the conservation value of each grid in the BTHr. The total area of biodiversity conservation priority areas was 36791.35 km², accounting for 16.94% of the total area of the BTHr. Conservation priorities were divided into three levels:the first-level conservation priority area (4611.57 km²), second-level conservation priority area (16045.79 km²), and third-level conservation priority area (16133.98 km²). These conservation priority areas are mainly distributed in the north of Hebei Province and Beijing. Most of them are dominated by forest vegetation and shrub vegetation. We suggest that these areas should be considered in the layout of national parks and ecological and environmental protection projects.