The experiment was designed that there were different wind speed (6, 9, 12, 15 and 18m/s), different wind-drift blowing (0.00, 1.00, 28.30, 63.28, 111.82 and 172.93 g cm^-1 min^-1), and five wind blowing times (10, 20, 30, 40 and 50 min) in spring, 2018 in Yantai, Shandong Province by using outdoor portable wind tunnel to understand the how physiological responses of Pinus thunbergii Parl seedlings to wind blowing and wind-drift blowing. The relative water content (RWC), malondialdehyde (MDA) contents, membrane permeability, osmotic regulators (soluble sugar, proline), and antioxidant enzyme activities (superoxide dismutase (SOD), catalase(CAT), peroxidase(POD)) were tested in the leaves of seedlings of Pinus thunbergii Parl under the previous treatments. The results showed that under treatment of wind blowing, with increasing of wind speed to 15m/s, the RWC was not changed, MDA content and membrane permeability increased slightly, while the proline content decreased, and the activities of SOD, CAT, and POD were slightly enhanced in the leaves of Pinus thunbergii Parl.. Under treatment of wind-drift blowing, with increasing of wind speed to 15 m/s, the RWC decrease, the membrane permeability and the contents of MDA and proline increased, and the activities of SOD, CAT, POD enhanced. At same wind speed, the value of each resistant physiological index under treatment of wind-drift blowing was higher than those under treatment of wind blowing in the leaves of Pinus thunbergii Parl. However, under wind blowing and wind-drift blowing at 18 m/s, the contents of MDA, proline, soluble sugar, and the activities of SOD, CAT, POD decreased with time extension to 50 min in the leaves of Pinus thunbergii Parl. It indicated that leaves of Pinus thunbergii Parl had stronger capacity of resistant dehydration, which was correlated with its ability of resistance to wind blowing. But cell dehydration induced by wind-drift blowing would be a main factor, which could not only promote the accumulation of proline and keeping on soluble sugar content to maintain water balance, but also activate antioxidant defense system to scavenge oxygen free radicals, and inhibit membrane lipid peroxidation to preserve the integrity of cell membranes. It suggested that Pinus thunbwould beergii had stronger capacity of osmotic adjustment and antioxidant defense system could be an important physiological adaptation mechanism for it to resistance to wind-drift blowing.