As the most important type of nature reserve in China, forest ecosystem nature reserve usually has complex and diverse protection objects. In order to regionalize its functions, the needs of various protected objects should be considered comprehensively. Evaluating the functional zoning of the forest ecosystem nature reserve is conducive to improving the protection effectiveness of it. In this study, we took the functional division of Henan Liankangshan National Nature Reserve, a forest ecosystem type nature reserve, as the research object. From December 2016 to December 2018, we conducted surveys of Line transect and infrared-trigger camera in this nature reserve to obtain the distribution point data of Reeves's pheasant and the related species. Combined with the collected environmental data, we preformed the distribution and the species interacting of Reeves's pheasant (Syrmaticus reevesii) by MaxEnt model. Furthermore, the spatial relationship between species and functional zoning of the nature reserve was analyzed, as well as the spatial relationship between the distribution of wild animals and the intensity of human disturbance in the nature reserve. Then, we evaluated the protection effectiveness of the Liankangshan National Nature Reserve existing functional zoning for the Reeves's pheasant and the species interacting of Reeves's pheasant. The results show that the area of high protection value area in this nature reserve is 33.84 km², and the area of high protection value in core area, buffer zone and experimental area account for 18.96%, 3.84% and 9.19% of the nature reserve respectively. The area ratio of high protection value area is relatively low in Liankangshan National Nature Reserve. The existing functional zoning of the reserve does not fully meet the need to protect the habitat of the Reeves's pheasant. The core area, buffer zone and experimental area are faced with different degrees of human disturbance, which poses a potential threat to the survival of the key protected objects in the nature reserve. Therefore, according to the present situation of protection value and human disturbance distribution, two different functional zoning optimization schemes of Reeves's pheasant were put forward. On the premise of not reducing the area of Liankangshan National Nature Reserve, increase the proportion of the suitable habitat area of Reeves's pheasant and related species in the core protection area, while reduce the intensity of human disturbance in the protection area. This study could provide decision-making basis for the protection of Reeves's pheasant in Liankangshan National Nature Reserve and provide guidance for the optimization of functional zoning of other nature reserve with wild animals as the protection goal.