Qinling-Daba Mountains is a transitional zone between the north and the south of China, which has obvious multi-dimensional zonal structure and complex geomorphological characteristics. The purpose of this paper is to study the trade-off and synergy of ecosystem services on different spatial scales which provides basic reference for the ecological protection of mountains, rivers, forests, fields, lakes and grasses among regions. We analyzed the trade-offs and synergies among Net Primary Productivity (NPP) of vegetation, grain production, soil conservation, habitat quality and water resources supply in the Qinling-Daba Mountains from 2005 to 2015 at the global, comprehensive regionalization, typical sample areas, and sample interval scales. The results showed that:① at a global scale, there was a synergistic relationship between water resources supply and habitat quality, soil conservation, NPP and grain production, NPP and habitat quality, soil conservation, habitat quality and soil conservation, and there was a trade-off relationship between grain production and habitat quality, soil conservation. ② At the regional scale, according to the geomorphological and climatic types of the comprehensive zoning, there were similarities and differences in the service function relationship within a specific region, coupled with different mechanisms affecting ecosystem services. It will lead to differences in the relationship between ecosystem services in the overall region. ③ At the typical sample area scale, there were obvious differences in climate type, vegetation type and geomorphological characteristics between the north and south slopes of the mountain, and the ecosystem services changed each other with the passage of time. ④ At the sample interval scale, there was a certain correlation between the trade-off/synergy relationship between the paired services and the sample interval scale, and the development trend of the trade-off/synergy relationship of different interval scales was different. It is of practical significance to study from large, medium and small scales, which can help decision makers to formulate corresponding ecological management strategies at appropriate spatial scales and promote eco-economic coordinated development.