Grasslands are one of the major types of terrestrial ecosystems and their soil respiration makes up one of the largest flux of carbon between their ecosystems and the atmosphere. Therefore, revelation of the responses of their soil respiration to global warming can be vital and will shed light on identifying and predicting the global carbon cycle. To simulate climate change, we used two open-top chambers (OTCs) T1 (0.5 m) and T2 (1.85 m) to manipulate warming Leymus chinensis systems and meanwhile observed the growth of Leymus chinensis. The soil respiration of these systems in both OTCs was monitored and the soil respiration rate was measured at the time t₁ (9:00-11:00), t₂ (13:30-15:30), and t₃ (17:00-19:00). The change in the soil respiration of heated Leymus chinensis systems was investigated to identify the main factors contributing to release of soil carbon to atmosphere and put insight into the relationship between the soil respiration and these main factors. The observations showed that T1 and T2 increased annual surface soil temperature by 2.14 and 4.03℃ respectively compared to control, but decreased annual soil moisture by 2.27% and 4.57% in the soil depth range between 0 cm and 10 cm. The results demonstrated that the manipulated heating significantly (p < 0.05) enhanced soil respiration through growth stages and soil respiration patterns changed over seasons. In grain filling, heading, and blossom stage, peak values of soil respiration rate were observed at t₁, t₂ and t₃ in the Leymus chinensis of the same growth day. The soil respiration rate did not change significantly at non-growth stages. The study also found that the soil respiration rate under different treatments had a positive exponential correlation to near-surface air temperature, surface soil temperature, and below-ground biomass. It also had a negative quadratic correlation with surface soil moisture and a positive quadratic correlation with above-ground biomass. This study reveals the change in release of soil carbon from Leymus chinensis systems to atmosphere when they were treated with manipulated warming, which can provide useful information for understanding how grassland ecosystems respond to climate change and sustainable development of animal husbandry.