The dramatic impact of global change and human activity has led to changes in ecosystem functions and services that are vital to human well-beings. Consequently,ecosystem degradation has become a serious problem globally. Comprehensive evaluation of the benefits of ecological restoration is an important way to measure the effects of implementation of restoration projects. In this study, China's key fragile ecological regions were selected as research objects. Four typical services were selected, including water production, soil conservation, food supply, and carbon sequestration, to construct a comprehensive evaluation index system for ecological restoration in the research area. Welch's t test was used to test restoration of ecosystem services in significant and insignificant regions and to comparatively analyze changes in the ecological restoration benefits in the study area. The results showed that from 2000 to 2015, the implementation of ecological restoration projects increased the overall ecosystem services of the fragile area by 20.86%.The comprehensive benefit changed from volatility to stability and stabilized around the mean value 0.55.The comprehensive benefit values of the karst area were the highest, reaching greater than or equal to 0.75. The changes in ecological system types were mainly the transformation between farmland, forest, grassland, and settlement ecosystems. The increase in forest and grassland ecosystems promoted the level of ecosystem service. The results of the t test indicated that ecological restoration led to an increase in comprehensive benefits and ecosystem services.