Analysis of the transformation of land use function and ecological environment response is a fundamental basis for the delineation and control of regional "production-life-ecology space" and ecological environment protection. Based on the remote sensing data in 1995, 2000, 2005, 2010 and 2015, we constructed a land use classification system of "production-life-ecology space" in the resource-abundant area in western Guangxi. Using transfer matrix, center of gravity transferring and ecological environment response model, the paper has analyzed the transformation characteristics of land use function and response of eco-environment in the study area in the past two decades. The results showed that: (1) the areas of ecological production space and life space of the research area were rising, while the production of ecological space and ecological space were declining. (2) The transfer distance of the center of gravity of ecological production space was the largest, followed by the center of gravity of life production space, and the transfer distance between the center of gravity of production ecological space and the center of gravity of ecological space was relatively small. (3) The ecological and environmental quality index of the research area decreased from 0.6337 in 1995 to 0.6323 in 2015, and the overall quality declined. (4) The urban ecological environment exhibited higher quality, while the critical mineral resource county and the agricultural production base presented lower quality, and the quality of ecological environment in key urbanization counties was the lowest.