Construction of ecological security pattern aims to identify important ecological regions of the study area and maintain their interconnection. It is considered as an important way to achieve the coordinated development at the regional scale. There are some technical difficulties to build ecological resistance surface in the construction of ecological security pattern. This paper took the Three Gorges Reservoir Area in Chongqing section (TGRAC) as a case study. Based on GIS, ecological sources were identified in terms of ecosystem services' importance and ecological sensitivity. The ecological corridors were identified through using minimum cumulative resistance model. The results showed as follows: (1) the area of ecological source was 19227.04 km², which accounted for 41.65% of the total area of the TGRAC. The ecological source area was mainly composed of forest land in the northeast and central part of the study area. (2) Compared with the night light index, the connectivity index corrected the resistance surface more obviously in the fragmented landscape areas and areas with strong human activities, and could also reflect the impacts of human activities to a certain extent. It showed that the connectivity index could modify the ecological resistance surface, and its correction effect was better than that of the night light index. (3) The total length of ecological corridors network in TGRAC was 1610.48 km, including natural corridors and resistance corridors. There were 30 strategic points, mainly located near cultivated land, residential areas, and waters. (4) The ecological connectivity of the study area was well, and it generally formed a spatial pattern of "one axis, three zones". However, corridors and blockage of strategic points were lack in some areas. Therefore, this paper proposed a restoration scheme to ensure the integrity of the ecological network in the study area.