Exploring the ecological service functions in the basin area is of great importance in efficient allocation of environmental resources allocation and rationally environmental policy design. Based on the model of the ecological service value proposed by Costanza, the Min River Basin is taken as the research object and the remote sensing images of 2006, 2011 and 2016 are used to extract information about the water, forest, grassland, cultivated land, impervious surface and bare land. By integrating ecosystem service value table, ecosystem service value estimation model and ecosystem service function trade-off coordination model, the ecosystem service value in the Min River basin and its trend were analyzed, then the trade-off synergy was explored. The results showed that the land-use system in the Min River basin significantly changed in 2006-2016, with increasing forest area, cultivated land area and impervious surface, as well as decreasing grassland area, water area and bare land area, especially with the most significant changes of water area. The overall value of the ecosystem services decreased from 101.12×10⁹ yuan in 2006 to 99.54×10⁹ yuan in 2016. The relationship between the ecosystem services in the Min River basin was mainly dominated by synergies in 2006-2016 and the share of synergies increased significantly during 2011-2016, while the synergy degree gradually decreased from 2011. Similarly, the results of the trade-off of ecosystem services in the Min River basin were also shown in the upper reaches during this period. It means that the upper reaches can be regarded as a "diffraction region" in the urbanization progress, and also treated as one of the key regions to effectively adjust the relationship between ecosystem services for the Min River basin.