The Jing-Jin-Ji region is located in the transition zone of Mongolian plateau and North China Plain, which is an important shelter for the ecological security of North China Plain. Based on field observation, remote sensing monitoring, the quantitative simulation and GIS spatial analysis, we constructed a set of ecological background maps of ecosystem structures and services in Jing-Jin-Ji region. We analyzed the spatial and temporal dynamics of ecosystem structures, sand fixation service, water regulation service, and soil retention service from 2000 to 2015. The impacts of climate change and ecological restoration projects on ecosystem service changes were discussed. The results showed that (1) farmland was the main ecosystem type in the Jing-Jin-Ji region. Urban areas increased significantly, while farmland areas decreased significantly. Forest, grassland and wetland area decreased slightly. (2) In 2000-2015, the average annual volumes of sand fixation, water regulation and soil retention were 5.61×10⁸ t, 74.58×10⁸ m³ and 7.98×10⁸ t, respectively. The ecosystem services in forest ecoregion were the highest among the four ecological-functional zones, and the ecosystem services in agricultural ecoregion were the lowest. (3) From 2000 to 2015, no significant increasing trends were revealed for the sand fixation service and water regulation service with increasing rates of 0.11 t hm^-2 a^-1 and 0.03×10⁴ m³ km^-2 a^-1, respectively. The soil retention service had a significant upward trend with 1.08 t hm^-2 a^-1. There were great differences of ecosystem service changes among four ecological-functional zones. All ecosystem services in forest ecoregion showed the increasing trends (P < 0.05 in some areas), while the sand fixation service in grassland ecoregion was no significant decrease. Unobvious trends of ecosystem key services were presented in other regions. The sand fixation service showed a significantly downward trend, and the soil retention service showed no significantly upward trend in urban and agricultural ecoregion. (4) Overall, the warming and wetting climate during the growing season, and the implementation of a series of key ecological restoration projects contributed to the increasing vegetation coverage and the improvement of the ecosystem services. However, rapid urbanization led to continuous expansion of buildup areas, which resulted in the degradation of vegetation coverage and ecosystem services.