The physicochemical properties of soil was directly affected by grazing and trampling of animal. In order to explore the main factors affecting soil physical and chemical properties by Père David deer grazing, parameters including water content, bulk density, salt content, organic matter, total N, P and K contents under different intensity of semi-free Père David deer disturbance were analyzed. The fieldwork was conducted in Dafeng Père David deer National Nature Reserve, Jiangsu province, China. The results indicated that (1) the contents of soil water and organic matter were negatively correlated with the increase of the grazing intensity. However, the contents of soil salt content, total N, P and K contents were positively correlated with the increase of grazing intensity. (2) The soil physicochemical properties parameters between the heavy grazing and the control plots showed significant differences (P<0.01). (3) The maximum value of total N, P and K contents was 1.56 g/kg, 0.95 g/kg and 13.43 g/kg in heavy grazing area, respectively. Especially, N content was the most remarkable parameter. (4) The soil salt content was up to 9.26 g/kg in heavy grazing area. Soil salinization was the main reason causing the habitat degradation in Dafeng nature reserve. The increasing of grazing intensity led to soil salinization and habitat degradation. The heavy salinized soil degenerated to bare land gradually.