The Life Community Theory holds that there is a general relationship between ecological factors, so the integrity and connectivity of ecological process should also be maintained in the construction of ecological security pattern. In this paper, six urban districts of Ningbo City were chosen as the research area, and the ecological source areas were identified by the combination of landscape connectivity evaluation and habitat quality assessment. In the construction of the resistance surface, the terrain, forest land and water body factors were selected to adjust the basic resistance surface set by the land use type. The ecological corridors were identified by the model of minimum cumulative resistance, and the ecological source areas, ecological corridors and ecological conservation areas were superposed to construct the ecological security pattern. The results show that the ecological source areas are 47.24 km² accounting for 1.30% of the total study area. The distribution of ecological sources is scattered, uneven in the east-west direction, and more in the eastern region. The total area of the ecological protection zone is 1191.67 km², accounting for 32.83% of the total study area. The total length of the ecological corridor is 519.32 km. The ecological corridors in the study area are distributed in a network, which effectively promotes the flow of material and energy. The application of neighborhood analysis method in the resistance surface setting highlights the relationship between different ecological factors, embodies the idea of Life Community Theory, and provides references for ecological protection and restoration in this area.