The mechanism of species coexistence is an important issue of ecological research. The spatial distribution pattern and intraspecific and interspecific relationships of Symplocos sumuntia and Symplocos lancifolia were analyzed in this paper. All Symplocos sumuntia and Symplocos lancifolia trees were divided into four diameter classes which represented four growth stages:sapling, small tree, medium tree and adult tree. The spatial distribution pattern of Symplocos sumuntia and Symplocos lancifolia were analyzed by single-variable pair-correlation function, and bi-variate pair-correlation function was used to analyze the intraspecific and interspecific interactions. The results showed that the diameter structure of Symplocos sumuntia and Symplocos lancifolia was basically the same, and two populations were growing populations. Symplocos sumuntia and Symplocos lancifolia populations were aggregated distribution characterized by the complete spatial randomness model, and gradually become to a uniform distribution; Under the heterogeneous Poisson model, the two species only presented aggregation in small-scale and showed a random distribution in the larger scale range. According to the intraspecific relationships, each growth stage was positive association of Symplocos sumuntia, and gradually changed into uncorrelated relations; in addition to the small trees and adult trees, the other two growth stages were positively associated with each other for Symplocos lancifolia. There was positive association in the small scale, and no association in other scales after excluding habitat heterogeneity. The sapling and small trees mainly displayed negative association of interspecific relationships, while no association for middle trees and adult trees. The results showed that Symplocos sumuntia and Symplocos lancifolia presented intraspecific aggregation and interspecific segregation patterns of the spatial configuration, and showed coexistence under the action excluded competition from the same genus. It would be beneficial to the stability of community structure and keep maintaining species diversity in subtropical evergreen deciduous broad-leaf mixed forest.