Aiming to the soil acidification and serious root disease of tobacco caused by continuous cropping, this study investigated the roles of Pseudomonas koreensis CLP-7 in soil pH, nutrients, enzyme activity and soil microbial community diversity of acidified rhizosphere soil of tobacco. The experiments were carried out in the field. The effects of pH, content of nutrients, soil enzyme activity were determined by chemical methods. The study of soil microbial community diversity was conducted with BIOLOG micro plate. The results showed that acidophilic P. koreensis CLP-7 significantly improved pH, the content of available K, available P, NH₄⁺-N, and NO₃^--N. The organic matter of acidified rhizosphere soil were higher than that of CK. The increase of organic matter content was the most significant. The urease and invertase activities of rhizosphere soil after adding CLP-7 were significantly higher than those in the untreated soil, but the catalase activity was not obvious. In 30 d of CLP-7 application, with the increasing time of CLP-7 application, the microbial diversity index of rhizosphere soil of tobacco showed obvious difference. At the 30th day of adding CLP-7, Shannon index, Simpson index, Richness and McIntosh index were reached the highest level, but the Pielou index changed a little. The results of principal component analysis of microbial carbon source metabolism showed that the soil microbes had significant differences in carbon source utilization after CLP-7 application, especially carboxylic acids and carbohydrates carbons. As the application time increased, the microbial utilization ratios of amino acids, carboxylic acids, miscellaneous, polymers and carbohydrates were significantly improved. It was indicated that CLP-7 was beneficial to improve the utilization of carbon sources in rhizosphere soil microorganisms in continuous cropping fields. This study showed that the application of acidophilic P. koreensis CLP-7 could improve the quality and microbial functional community diversity of acidified tobacco soil and create a soil ecological environment that was favor of tobacco growth, which had a greater potential to application in microbial bioremediation and tobacco green control.