Wushengguan Valley is located at the intersection of the Palaearctic Realm and Oriental Realm, its unique transition zone has formed abundant plant resources, and the plant landscape conservation regulation contributes to keeping the site stable. Based on the unmanned aerial vehicle technology and application of GIS, we obtained the natural resources, landform, hydrology, field of vision and other characteristics of Wushengguan Valley, as well as ecological sensitivity (conservation level) and plant niche type distribution. The results show that: (1) The overall topography of the site gradually increases from northwest to southeast, and it is a typical valley type; (2) Various slope position changes bring different changes in solar radiation and the visual field along the road, mainly showing that the amount of solar radiation on both sides of the road in the middle of the study area is low and the interference may be large; (3) Highly sensitive regions are mainly distributed on the south side of the research area road, areas with high soil fertility are mainly distributed in broad-leaved forest areas, hydrophilous plants are suitable for distribution on the two sides of the site road, and the sciophytes are mainly mostly ideal for distribution on the south side of the road. According to the analysis results, combined with the theories of niche, landscape heterogeneity, and edge effects, from the four aspects of split range control, zone protection, vegetation patch transformation and vegetation selection, and sustainable management and conservation, we propose a systematic strategy for plant landscape conservation regulation in Wushengguan Valley. The research has important significance in the protection of natural landscape resources and the sustainable development of plant landscape resources. In the future, further explorations can be made on the small-scale operability.