In this study, the effects of warming (13℃, 19℃ and 25℃) and yak excreta (feces, urine) addition on soil properties and enzyme activity of marsh soil were studied by laboratory incubation experiment. The results showed that: (1) Warming induced an overall reduction in pH of marsh soil (P<0.05). The inorganic nitrogen content of marsh soil was decreased at 19℃ (P<0.05), but there was no significant difference among temperature treatments of other soil properties (P>0.05). The effects of warming on soil enzyme activity with different excreta treatments were various significantly. (2) The effects of excreta addition on the physicochemical properties of marsh soil were different significantly (P<0.05). Yak feces addition significantly increased water content, pH, TOC and available phosphorus content (P<0.05), but decreased nitrate nitrogen content of marsh soil (P<0.05). Yak urine significantly increased water content and inorganic nitrogen content of marsh soil (P<0.05). Yak feces addition significantly promoted the activities of sucrase, urease and catalase (P<0.05). In contrast, Yak urine significantly promoted urease activity (P<0.05), but had no significant effect on other enzyme activities (P>0.05). (3) The interaction of warming and excreta addition had significant effects on TOC (P<0.05), pH, inorganic nitrogen content (P<0.01), sucrase (P<0.05), urease, acid phosphatase and catalase (P<0.01) activities. This study illuminated the effects of warming, yak excreta addition and interaction on the physicochemical characteristic and enzyme activity of marsh soil, which provided theoretical basis for study of the effect of grazing disturbance on wetland ecosystem with the climate warming.