Identifying the differences in environment-sensitive zooplankton community characteristics and influencing factors among different types of landscape waters is critical for maintenance of biodiversity and protection of aquatic ecosystem. Zooplankton was sampled from 35 sites in 8 typical landscape waters in Shanghai and in situ physicochemical variables were measured in May 2014. A total of 40 species of zooplankton were recorded in the study area, of which 29 species were recorded as Rotifera, 7 species as Cladocera, and only 4 of them as Copepoda. Cluster analysis showed the zooplankton communities in all sampling sites were clustered into four groups:natural fresh-water lake (Lake Dianshan), man-made lake (Lake Dishui), waters in AAAA grade parks (Gongqing Forest Park and Guyiyuan Park), and waters in ordinary comprehensive parks (Century Park, Heping Park, Changfeng Park, and Chejing Park). Zooplankton species in the two types of park were richer, of which about 40% were endemic species. While there was only 1 endemic species (Harpacticoida) in Lake Dianshan, and three endemic species (Bdelloidea) in Lake Dishui. In terms of species similarity, the natural Lake Dianshan had the highest similarity (37.5%) with the man-made Lake Dishui, followed by ordinary parks (33.3%), and AAAA grade parks (20%). The species similarity between the latter two was higher (27.8%), while Lake Dishui had a lower similarity with the latter two (18.5% and 12.0%). There were only three taxa (Brachionus angularis, Keratella cochlearis and Copepoda nauplii) detected in all types of waters. The relative abundance of crustaceans in waters of AAAA grade park (88.8%) was much higher than that in other waters. The dominant species in waters of AAAA grade parks were composed of three large-size zooplankton species (crustaceans). The dominant species in waters of ordinary comprehensive parks were composed of four small-sized zooplankton species (rotifers). The community structures in both Lake Dianshan and Lake Dishui were simple. Redundancy analysis showed water temperature, transparency, pH, and chemical oxygen demand were significant factors affecting zooplankton community structure in landscape waters.