Methyl jasmonate (MeJA) is closely related to interplant communication. It can act as a volatile signal to travel through the atmosphere to induce plant defense. In order to explore the concentration and distance effects of MeJA volatile on neighboring plants' resistance against herbivore insects, we placed tomato plants at distance of 60 cm, 120 cm, 180 cm, 240 cm and 300 cm from the entrance, and then sprayed MeJA at the entrance of the greenhouse (length 4 m×width 1 m×height 1 m), activities of peroxidase (PPO), polyphenol oxidase (POD) and lipoxygenase (LOX) of leaves of tomato plants at different distance away from MeJA odour as well as Spodopter litura larvae mass gain fed on plants at distance of 60 cm and 300 cm were tested. Our results showed that airborne MeJA volatilized from 1 mmol/L and 10 mmol/L MeJA could significantly induce PPO, POD and LOX activities in the leaves of tomato plants, and the induced effects were most prominent on tomato plants placed at the distance of 60 cm from 10 mmol/L MeJA volatile, but the inductive effects decreased with the increasing distance. S.litura larvae fed on plants placed at distance of 300 cm from 1 mmol/L and 10 mmol/L MeJA volatile gained more mass than those fed on plants placed at distance of 60 cm, while mass of larvae fed on 10 mmol/L MeJA treated group were the least at the same feeding time. Collectively, these results indicate that volatile MeJA resulted in significant concentration and distance effects on tomato plants' resistance against S.litura.