Exploration of the accessibility of ecological space recreation service (ESRS), its driving forces and unfairness are very important to optimize the land use management and improve the well-being of residents in urban agglomeration area. Taking the Pearl River Delta Urban Agglomeration(PRDUA) as a case, the modified Two-step Floating Catchment Area method (2SFCA) was used to evaluate the supply-demand matching pattern of the ESRS and its influencing factors. The results showed that: (1) the accessibility of ESRS decreased from the marginal zone to the core region. (2) There was greater unfairness of accessibility of ESRS in the PRDUA. (3) The spatial pattern of accessibility was affected by both natural and human factors. The natural factors included elevation, ecological recreation space distribution, while human factors included population density, industrialization and urbanization pattern, urban planning and policy. The pattern of rapid urbanization and industrialization in the PRDUA led to the environmental unfairness. The low supply of ecological recreation service in the core area suggested that the landscape structure optimization of urban agglomeration should focus on the ecological restoration, optimize the spatial distribution of ecological recreation spaces, and promote the eco-city model when constructing new cities or new districts to realize the coordinated development of ecological recreation space, living space and production space.