It cannot be ignored that the impact of rapid development of tourism activities on reproduction and population expansion of wild animals. Most Galliformes nest on the ground and are susceptible to human activities. To investigate the impact of tourism activities on the nesting rate of Galliform species, the ground artificial nests were placed in Dongzhai National Nature Reserve, Henan Province for simulation test from March to May, 2018. We analyzed whether the experiment rounds, nest density, visitors flow rate, and vehicle flow rate representing the intensity of tourism activities had relationship with the nest survival rate. We also analyzed the influence of habitat factors such as vegetation type and altitude on the artificial nesting rate. We found that there were significant differences in altitude and visitors flow rate between the breeding success nests and failure nests. The breeding success nest were located in low altitude and large visitors flow rate area. The apparent survival rate of artificial nests was significantly higher in areas with high visitors flow rate and low altitude. The influence of visitors flow rate on nest survival rate is a dominant factor. In this study, the apparent survival rate of the artificial nest in the second round experiment was significantly lower than that in the first round experiment. Moreover, the composition of the artificial nest predators also showed significant differences in different test rounds. The first round experiment was mostly birds, and the second round experiment was mammals. Collared crow, raccoon dog, and wild boar were the most threatening predators in this study. The results suggest that the moderate tourism may affect the reproduction of Galliformes. Therefore, it is suggested that the wildlife distributed in this area and priority protection policy should be taken into account in tourism activities. The intensity of tourism activities should be controlled in order to avoid adverse effects on wildlife and strengthen the protection of habitats.