Slope runoff is the most important soil erosion force in karst areas, the properties of which in a typical karst trough valley are of great significance for soil erosion prevention and for groundwater resources management. In this study, four standard runoff plots with different land-use patterns were set in a typical karst trough valley located in the Zhongliang Mountain, Chongqing. Land-uses in the trough valley, were farmland, orchard, bamboo land and forest. Hydrological characteristics, including precipitation, surface runoff, soil-layer flow, fissure flow and soil moisture content, were monitored simultaneously, and then slope runoff properties in the typical karst trough valley were determined by slope runoff plots. The results were:(1) In the four runoff plots, the total runoff coefficient (3.1%-5.2%) was significantly lower than that of non-karst areas (approximately 20%), and the total slope runoff of the four kinds of land-use decreased in an orderly way, with farmland being observed to have the greatest amount (3696.9L), followed by orchards (3657.2L), then bamboo land (2922.9L), and finally forest (2211.1L). (2) Surface runoff was the dominant runoff form in the four runoff plots, and this was followed by soil-layer flow and fissure flow. (3) Precipitation factors and antecedent soil moisture content together affected surface runoff discharge; however, precipitation factors were the predominant factor. Four precipitation factors were involved in the surface runoff formation analysis, in which the maximum intensity of continuous rainfall in 15 minutes was the predominant factor in farmland and orchards, and precipitation was the predominant factor in forests and bamboo land. Meanwhile, the antecedent soil moisture content had relatively greater impact on farmland, followed by forest land and then orchards, than bamboo land.