As the process of urbanization in China is accelerating, the biodiversity in wetland parks will also be affected. Scientific and rational assessment of biodiversity provides an important reference value for the construction or maintenance of the same types of wetland parks in cities. In this study, we selected the 5-year-old Liupanshui Minghu National Wetland Park in Guizhou Province and analyzed its biodiversity from habitat and species diversity. The former categorizes the "habitat units" in wetland parks into categories:punctual elements, linear elements, and planar elements, and calculates the corresponding area. The latter uses a sample method to investigate the number of species of vascular plants in the park and uses the Shannon-Wiener index for calculation, based on the diversity index of the two. ArcGIS overlay analysis was used to obtain the spatial coupling relationship between habitat diversity and species diversity. Subsequently, the corresponding habitat elements that affected the variation of species diversity were analyzed and optimized protection measures were proposed.