Freeze-thawing affects the soil gross nitrogen (N) mineralization in forest ecosystems, but is lack of the quantitative characterization. The response mechanism of N mineralization rate and conversion to the freeze-thawing process is not clear. In this paper, ¹⁵N pool dilution technique with situ and indoor incubation methods was used to analyze the dynamic process and accumulation of gross N mineralization during the freeze-thawing period (from November 1, 2017 to April 15, 2018) of three community types of soil in woodland, bushland, and grassland in Tianshan Mountain. We also analyzed the relationship between soil gross N mineralization rate and soil temperature, moisture content and microbial biomass N (MBN) during freeze-thawing. The results showed that:(1) the gross N mineralization rate, net N mineralization rate and MBN content have been affected by the freeze-thawing process and the community types significantly (P < 0.01). The gross N mineralization rate in autumn and spring freeze-thawing period was higher than that in the constant frozen period. (2) The cumulative gross ammonification accumulation of woodland, bushland, and grassland communities in the whole period was 163.9, 88.3, 103.4 kg N hm² a^-1. The ratio of gross ammonification accumulation during freeze-thawing period accounted for about 66% of that accumulated in the whole period. The gross nitrification was 55.4, 41.6 and 47.1 kg N hm² a^-1, and the ratio was about 77.4%. (3) The gross N mineralization rate, net N mineralization rate, and MBN rate have been affected by soil temperature and moisture significantly. As soil temperature increased, gross ammonification rate (only woodland and bushland) increased significantly (P < 0.05). When soil moisture content increased, net ammonification rate (bushland), net nitrification rate (bushland) decreased significantly (P < 0.05). By revealing the soil gross nitrogen mineralization rate (gross ammonification rate and gross nitrification rate) and the cumulative amount of conversion, the response to the freeze-thawing process can provide a theoretical basis for the biogeochemical process of forest soil in Tianshan Mountain.