Natural ecological spatial management is an important part of national spatial management, and it's also a hot topic of eco-environmental research in China and abroad. Under the background of the domestic natural ecological spatial use management temporarily being in the pilot stage and the method of management needing to be further explored,this paper aims to provide a reference for the reasonable protection of natural ecological resources and promote the healthy and orderly development of natural ecosystems based on natural ecological spatial management zoning in Jiangxi province.After evaluating of ecosystem services importance and ecological sensitivity, this paper discussed methods of natural ecological spatial management zoning, in Jiangxi province by GIS spatial analysis technology.The results show that the area of the natural ecological space is 117924.67 km², accounting for 70.66% of the total area of Jiangxi province, are divided into three types by the method of two-dimensional judgment matrix analysis.Different approach of measurement is suitable for different types of areas.Among them, the ecological protection and ecological restoration are the main task for high-importance and high-sensitivity area. In the medium-importance and medium-sensitivity area, it is expected to rely on the regionally ecological resources to carry out ecological operation activities with main purpose of maintaining and improving the ecosystem service functions.For the low-importance and low-sensitivity area, the moderate development of production and construction activities was allowed in certain scale, to reduce pollution discharge and strengthen regional ecological function. However, this can only be done without destroying the structure and function of the ecosystem.Besides, the research indicated that all 16 national nature reserves were in the range of high-importance and high-sensitivity area.The natural ecological spatial management zoning and the general land use planning of Jiangxi province complemented each other. Natural ecological spatial management zoning that based on the ecosystem service function and ecological sensitivity, could provide new ideas for natural ecological spatial management, thus effectively promoting the implementation of control work.