Phenolic acid allelochemicals are some of the most important agents to cause continuous cropping obstacles. As a perennial woody plant, the continuous cropping obstacles of Wolfberry (Lycium barbarum Linn.) are serious, and their contributed factors are complicated. To determine the effects of phenolic acids on Wolfberry, the allelopathic inhibition of 22 phenolic acids on the germination of wolfberry was quantified, and comparative molecular field (CoMFA) analysis was used to elucidate the relationship between allelopathic activities and structural characteristics. The results showed that half maximal inhibitory concentrations (IC₅₀) were in 39.94-115.97 mg/L. The CoMFA indicated that the allelopathic activities increased when substituents had large volumes at the 1/2/3/4-positions of the benzene ring, and/or a negative charge at the 1-position of the benzene ring. These results provide basic data for evaluating the allelopathic effects of phenolic acids on Wolfberry.