To clarify the composition and diversity of pollen plants of Megachile strupigera, the bee breads from four different types of sample plots, including Shuibei Town, Xinyu City (XYSB), Geping Town, Ji'an City (JAGP), Shadi Town, Ganzhou City (GZSD), and Qiyunshan Nature Reserve, Ganzhou City (GZQYS), were collected by artificial trap-nest and analyzed by cloning and sequencing. In total, 9 families, 10 genera, and 15 species were annotated. Vitex negundo and Vitex quinata were the dominant species. According to the indexes of Chao1, Shannon-wiener, and Simpson, the richness and diversity of pollen plants from the four sample plots were GZQYS > GZSD > XYSB > JAGP. Furthermore, each sample plot had a much higher species richness in early July than that of in mid-August. The significant differences and diversities of pollen plants among the four sample plots showed that different styles of forest land had significantly different effects on the diversity of pollen plants of M. strupigera, which would decrease with the increasing degree of artificial disturbance. For each sample plot, the diversity of pollen plants presented a declining tendency within the two time periods. However, the most abundance plant species did not change, which was not affected by seasonal flowering plants. Therefore, the main pollen plants of M. strupiger are V. negundo and V. quinata, which would play an important role in maintaining the population stability of M. strupigera.