The present study investigated the quantity and spatiotemporal patterns of Brevipalpus obovatus Donnadieu and its natural enemies in the tea garden of "Wuniuzao" and "Baihaozao" in Hefei suburban district, China in 2015 and 2016. The reasons for differences in the relationship between the same natural enemies and the pest species in the two years were evaluated. The ranges of spatial dependence of natural enemies were calculated by the method of geostatistics, and the spatial relationships among enemies and pests were analysed using grey relational analysis and ecological analyses. The four major enemies that showed close relationship with Brevipalpus obovatus Donnadieu in the tea garden of "Wuniuzao" were Erigonidium graminicolum, Tetragnatha squamata Karsch, Oxyopes sertatus, and Neoscona theisi, and in the tea garden of "Baihaozao" were Misumenops tricuspidatus, Neoscona theisi, Tetragnatha squamata Karsch, and Tetragnatha maxillosa. Furthermore, T. squamata, N. theisi, E. graminicolum, and T. maxillosa were closely associated with B. obovatus in both gardens in 2015, whereas O. sertatus, M. tricuspidatus, T. squamata, and E. graminicolum were closely associated with B. obovatus in both gardens in 2016, and T. squamata and N. theisi were included among the main natural enemies of both tea gardens during both years. These results provided an important foundation for further studies related to protecting natural enemies and optimizing control strategies of B. obovatus in tea gardens.