Scientific evaluation of the importance of biodiversity conservation function is an important part in process of the ecological protection red line delineation. At present, Net Primary Productivity (NPP) method is widely used in delineating the ecological protection red line because it needs less factors and simple calculation. However, the application of species distribution model is less because it needs more factors and complicated calculation. We applied Maximum Entropy (MaxEnt) model and NPP method respectively to evaluate the importance of biodiversity conservation function in Panjin, and then assessed the two methods. The results showed that the NPP method could not cover all important areas of biodiversity, and its results did not match the actual distribution of the birds because it was influenced by high NPP value of crops. Compared with the results of the NPP method, the MaxEnt model could not only get more accurate results, but also the results were more consistent with the actual distribution of the birds in Panjin. Finally, using the MaxEnt model method, the area of the biodiversity conservation function ecological protection red line in Panjin is 1050.17km², accounting for 25.85% of the land area of Panjin City. The area basically covers the habitats and migration paths of all key rare wild species such as Oriental White Stork, Red-crowned Crane, Black-billed Gull, and Aurora. This study has important guiding significance for the delineation of the ecological protection red lines. The results can provide scientific support for the protection and management of rare and endangered birds in Panjin.