The alpine meadow is the main type grassland ecosystem which has several unique characteristics. Slope direction is one of the important topographic factors in the alpine meadow which plays an important role in the spatial differentiation of soil properties. The difference in soil nutrients and microorganisms between different slope directions may lead to different landscape qualities, and the difference in landscapes will determine the nature of the soil which finally affects the vegetation structure and growth. Therefore, understanding the role of slop effects in driving soil microbes and functions is essential for formulating sustainable ecosystem management and conservation policies. The aim of the current study is to clarify the spatial distribution pattern of grassland soil properties under different slope directions and to provide a basis for the precise restoration of degraded grassland. The soil physical and chemical properties and microbial quantity changes in the degraded meadow of the alpine meadow in the eastern margin of the Qinghai Tibetan Plateau (N, northern slope; NW, northwest slope; W, west slope; E, eastern slope; NE, northeast slope; SW, southwest slope; S, south slope) were studied. The results showed that the soil organic matter, carbon and nitrogen ratio, and total nitrogen content decreased from north to south (N to S). The total phosphorus content and pH value of the soil were not significantly differed between the seven slopes. The content of total phosphorus in the E slope was the highest ((2.83±0.95)g/kg), and the content of the NW slope was the lowest ((2.07±0.12)g/kg); the counts of soil bacteria, fungi, and actinomycetes fluctuated with slope differences. The count of bacteria ((13.0×10⁵±1.0×10⁵)cfu/g), fungi ((14.0×10³±0.0)cfu/g) and actinomycetes ((24.0×10⁴±1.0×10⁴)cfu/g), was the lowest in the NW slope, highest in the E slope for bacteria ((85.5×10⁵±2.5×10⁵)cfu/g) and actinomycetes ((209.5×10⁴±4.5×10⁴) cfu/g), and on the S slope fungus ((24.0×10³±0.0)cfu/g) was the highest. The regression analysis showed that with the increase of soil water content, organic matter, C/N ratio, and total nitrogen content, the counts of soil bacteria and actinomycetes decreased significantly from the gradient of the N slope to the S, and there was no significant influence on the count of fungi. The change in soil pH value and total phosphorus had no significant influence on the number of microbes (P > 0.05). It could be concluded that soil nutrient contents were different under different slope directions. When the soil nutrient content was high, the demand for plant growth may be given priority. Therefore, during the period of high soil nutrient contents, the count of microorganisms remain relatively small.