In order to analyze the close degree of spatial following relationship, reason for aggregation and aggregation range between Fulgoroidea and their natural enemies under different cluster size conditions, and to provide scientific basis for evaluating dominate natural enemies of Fulgoroidea and determining the quadrats size.The block quadrat variance analysis, grey correlation analysis, aggregated-intensity index method of spatial patterns, mean method of population aggregation and ρ index method were used to analyze Fulgoroidea and their eight natural enemies under different cluster size conditions in "WuNiuZao" tea garden in Hefei city, Anhui province. The quadrats numbers at the peak of mean variance of Fulgoroidea and their eight natural enemies were analyzed by correlation analysis. The results showed that the first three natural enemies that closely follow the Fulgoroidea in space were Neoscona theisi(0.8010)、Erigonidium graminicolum(0.7617)and Misumenops tricuspidatus(0.7613). When there were 1, 2, 4 or 8 basic quadrats in the poly-block, with the increase of basic quadrats number, the diffusion coefficient C-value of aggregation pattern increased and the C-value of uniform or random patterns decreased. And the aggregation degree in space of Fulgoroidea and their natural enemies were not significantly different. The average population aggregation were greater than 2 in most of λ, indicating that the gathering was caused by the pest themselves, and the absolute value of λ was increasing with the number of basic quadrats in the poly-block. The smallest scope of individual population aggregation which was evaluated by using ρ index of Fulgoroidea with different sizes of poly-blocks was four basic quadrats with 16m² in this study. It provided a scientific basis for the determination of quadrats size during sampling. The block quadrat variance analysis was a simple and practical method to analyze the spatial relationship between pests and natural enemies.