The population of Sichuan snub-nosed monkey (Rhinopithecus roxellana) in Hubei Province occurs in the eastern most edge of species distribution; it has a small population size, genetic isolation, and low genetic diversity, which makes it more vulnerable to environmental changes. Focusing on the Shennongjia Area, we built a species distribution model to identify suitable habitat and achieve resistance surface for movement. We then used circuit theory to analyze landscape connectivity between suitable habitats. To investigate the influence of human disturbance on the moving path of the monkey and the connectivity between and within subpopulations, we combined scenario analysis, i.e., the scenario of road and tourism activity, with least cost model and circuit theory. Using the CDPOP software, we simulated the influence of road on the genetic diversity of monkey in 150 a. The suitable habitat aggregated in Shennongjia, and multiple potential dispersal areas between habitat patches were identified. The road changed the optimal route of monkey between the northern and southern suitable habitats. The tourism activity had no obvious influence on the moving path of the monkey, but resulted in a certain avoidance effect on the distribution of monkey with a distance of 100 m. The road significantly affected the connectivity within the Jinhoulin subpopulation and between all subpopulations, and the tourism activity had not significant effect on the connectivity of monkey. The genetic diversity of meta-population in 150 a exhibited a declining trend, and the roads will likely exacerbate the magnitude of the decline in genetic diversity. According to these results, we put forward some suggestions for the conservation of snub-nosed monkey. The results possess the reference value and the importance to protect this species and others.