Taking Zhoumai 18 as research materials, 252 and 254 as tested strains, wheat treatment group with salt stress, bacterial treatment group, and the control group used by artificial climate culture method were cultivated for 7d, 14d, and 21 d. The content of proline (Pro) and malondialdehyde (MDA) of wheat seedlings treated with inoculation and those of wheat under salt stress were determinated. The results showed that Pro content of salt stress group firstly rose then falled with the increase of culture time and salt concentration under salt stress environment. In the prophase and metaphase, contents of wheat Pro under salt stress gradually accumulated and improved the resistance of wheat. In the anaphase, Pro contents declined to certain degrees compared with those in metaphase, which indicated that wheat gradually adapted to salt environment. With increasing salt concentrations, wheat in various groups of salt stress exhibited different levels of resistances to salt environment at different stages of cultivation. During each period, there was a threshold of wheat resistance to salt concentration. When salt concentration was greater than such threshold, Pro content declined, and wheat resistance to salt was lessened. After inoculation with endophytes, higher Pro content of wheat improved the ability of wheat resistance to salt stress, and its respair effect of 14 d was the most obvious. When the treatment group inoculated with 252 and 254 respectively under the concentration of salt, their Pro content were higher 133.48% and 91.48% for 200 mmol/L and 250 mmol/L than those of salt stress group. MDA content of wheat with salt stress group was first drop and rose with time prolong, but Pro content changes had inverse trend. During the prophase and metaphase, in various groups of salt stress, lipid peroxidation degrees of wheat cellular membrane were not high, which brought about no severe threat to the normal metabolism of wheat. During the anaphase, MDA content increased and wheat in various groups of salt stress gradually withered. Due to the accumulation of reactive oxygen species in cells, some ingredients would be involved in severe reactions. After inoculation with endophytes, endophytes reduced the degree of lipid peroxidation of wheat cellular membrane, enhanced the survival rate of wheat, and promoted the growth of wheat seedlings. MDA content were lower than that on the 21d in salt stress group, and remediation effect was significant.