Regarding the ecological environmental integration of the Beijing-Tianjin-Heibei urban agglomeration, the combination of administrative, Tessellation polygon, and minimum accumulative resistance boundaries based on the minimum cumulative resistance model was used to improve the traditional evaluation method of the ecosystem service value based on administrative boundaries, to estimate the actual ecosystem service value of 13 cities in the Beijing-Tianjin-Heibei urban agglomeration. The results showed that (1) the Tessellation polygon and minimum accumulative resistance boundaries of biodiversity conservation and cultural service corresponding to the 13 cities are relatively different from the administrative boundaries; (2) the total ecosystem service value of the Beijing-Tianjin-Hebei urban agglomeration is 454.481 billion yuan, and the actual ecosystem service value obtained by the 13 cities are very different, showing a downward spatial trend from North to South; (3) compared with the estimation results of the traditional method, the actual ecosystem service values acquired in Beijing, Shijiazhuang, Chengde, Hengshui, Langfang, Qinhuangdao, Tangshan, and Xingtai cities increased. Among them, Tangshan City had the largest increase of > 5.65 billion yuan, whereas the actual ecosystem service value obtained by the other cities decreased, with the largest decrease in Tianjin, which exceeded 9.8 billion yuan; (4) the results of the sensitivity analysis showed that the change of the total ecosystem service value of the Beijing-Tianjin-Heibei urban agglomeration was stable to the value of ecosystem service; therefore, the estimation method was effective and the research result was credible.