Ecological assets are an important part of natural resource assets; therefore, ecological asset accounting will be of vital significance to the preparation of natural resource balance sheets. Based on the assessment of the quantity and quality of ecological assets, which included forests, brushwood, grasslands, and wetlands, we obtained the comprehensive index of ecological assets. Furthermore, we prepared the ecological assets physical quantity balance sheet and physical quantity of increase and decrease statement. The results showed that the forested area of Hinggan League increased 1.1%, and the area of grassland and wetland decreased 1.7% and 3.8%, respectively, during the 2000s. However, the quality of forest and grassland improved significantly; therefore, the comprehensive index of ecological assets increased 2.16%. Use of ecological asset accounting could be an effective tool to inform the governmental officials whether they meet the requirements of ecological protection, which will promote the construction of the ecological civilization of the Hinggan League.