The aim of this study was to investigate the characteristics of the spatial ecological niche of the Bacillus-like genera in a microbial fermentation bed (MFB) used for pig raising. To understand the ecological relationship between the environment and Bacillus-like genera, and thereby clarify the functions of pig feces degradation, odor elimination, pig disease prevention, and resource utilization, the random sampling method was used to obtain a total of 14 samples from the upper (0-20 cm) and under (40-60 cm) layers of the MFB. Nutrition condition analysis and metagenome sequencing methods were used to analyze the nutrition characteristics of the litter samples (organic matter, total nitrogen, humic acid, crude fiber) and growth conditions (moisture, pH), with identification of the Bacillus-like genera and determination of their relative abundance (reads). Cluster analysis, correlation analysis, spatial distribution analysis, and ecological niche width and overlap were used to reveal the characteristics of the spatial ecological niche and the relationships between it and other factors. A total of 24 Bacillus-like genera within six families were identified in the samples from the MFB (including two non-Bacillus genera), of which the genera Ammoniibacillus, Desulfuribacillus, and Tuberibacillus were all newly recorded genera in China. The three genera with the highest relative contents (reads) in the measured ecological niche were Bacillus (reads=8020), Lactobacillus (reads=4565), and Tuberibacillus (reads=1418), respectively. The total numbers of the Bacillus-like genera showed no significant differences (P > 0.05) between the upper and under layers of the ecological niche in the MFB. However, differences in the Bacillus-like genera were found at the level of their subcommunity structure:the top five genera with the highest contents were Bacillus (532.86), Lactobacillus (480.43), Geobacillus (88.86), Gracilibacillus (70.00), and Paenibacillus (40.86) in the upper layer, and Bacillus (612.86), Tuberibacillus (188.57), Lactobacillus (171.71), Paucisalibacillus (60.00), and Ureibacillus (46.71) in the under layer. The results showed that the five highest ecological niche breadth values of Bacillus-like genera were those of Bacillus (10.5159), Ornithinibacillus (8.6094), Paenibacillus (7.8463), Oceanobacillus (6.9927), and Rummeliibacillus (5.7417). The results indicated that the Pianka measurement values of the spatial ecological niche overlap between any two Bacillus-like genera ranged from 0.00-0.99, some of them had a high overlap, such as Gracilibacillus and Ammoniibacillus, while some of them had almost no overlap, such as Desulfuribacillus and Aneurinibacillus. Moreover, there existed a relationship between the ecological niche breadth and ecological niche overlap:the genera with a wide ecological niche breadth, such as Bacillus, had niche overlap values from 0.20-0.80 with the other genera, while those with a narrow ecological niche breadth, such as Geobacillus, had niche overlap values under 0.20 or over 0.80.