We determined ozone (O₃) uptake rates of the dominant tree species (Pinus manssoniana, Castanopsis chinensis, Schima superba, and Machilus chinensis) in a natural mixed conifer-broadleaf forest based on sap flow measurements and environmental monitoring. On a diurnal scale, the ozone uptake flux (FO₃) by the dominant tree species had a unimodal pattern, and that the maximum flux appeared earlier in the wet season (April-September) than in the dry season (October-March). Compared to the wet season, the time of the maximum flux in the dry season was delayed. Seasonally, the maximum O₃ concentration occurred in the wet season (48.94 nL/L). Canopy stomatal conductance for O₃ (GO₃), FO₃, and annual accumulative stomatal O₃ uptake (AF_st) in the wet season were significantly higher than in the dry season (P < 0.01). Machilus chinensis had the highest O₃ uptake capacity among the four species considered. The maximum FO₃ was 1.11 nmol m^-2 s^-1 in the dry season and 1.71 nmol m^-2 s^-1 in the wet season. GO₃ decreased with increasing vapour pressure deficit (VPD) in all the studied species. At photosynthetically active radiation (PAR) > 1500 umol m^-2 s^-1, GO₃ and FO₃ for all species declined at higher PAR. Annual AF_st exceeded the threshold that could be tolerated by forest trees, suggesting a potential O₃ risk for mixed conifer-broadleaf forest in Dinghushan.