An investigation was conducted in autumn 2014 and spring 2015 in order to learn the temporal and spatial distributions of the zooplankton community, as well as its correlation with environmental factors in Qinzhou Bay. Results show distinct seasonal differences in the community. In autumn, 87 species of zooplankton were detected with 5 dominant species, Acartia pacifica, Evadne tergestina, Lucifer hanseni, Sagitta bedoti, and Macrura larvae; whereas in spring, 48 species were found with 2 species dominant, Calanus sinicus and Acartia pacifica. The average abundance and biomass of zooplankton was greater in autumn (528.92 ind/m³ and 110.60mg/m³,respectively) than in spring (48.30 ind/m³ and 68.64mg/m³,respectively). The Shannon-Wiener diversity index was higher in autumn (2.22) than in spring (1.70).Spatial distribution wise, the abundance, biomass, and diversity of the zooplankton species were higher in the outer bay than that in the inner bay. Furthermore, according to our MDS analysis, composition of the zooplankton community had high similarity in the inner bay in autumn, while the same was observed in the outer bay in spring. The results of the correlation analysis showed that salinity and nutrients were the main environmental factors that affected the zooplankton community. Comparing our results with historical data from 2011 to 2012, we found that the composition of the zooplankton community in Qinzhou Bay showed trends of simplification and had decreased in abundance, which could indicate that high density shellfish culture and eutrophication affected the zooplankton community in Qinzhou Bay.