Abstract:To protect and use of natural enemies and provide scientific sampling methods, in this study, the spatial relationships among the natural enemies of the insect pests Toxoptera aurantii Boyer, Empoasca vitis Gothe, Breuipalpus oboyats, and Monolepta hieroglyphica were investigated in the tea garden of "WuNiuZao" in the Hefei suburban district, China in 2015 and 2016. The reasons for differences in the relationship between the same natural enemies and the pest species in the two years were evaluated. The ranges of spatial dependence (RSDs) of natural enemies were calculated using a geostatistics method, and the spatial relationships among enemies and pests were analyzed using grey relational analysis. A greater relative degree indicates a closer following space about the relationship between natural enemies and pests. The relational grade analysis showed that the three main enemies Neoscona theisi, Tetragnatha maxillosa and Clubiona japonicola Boes. et Str had a close relationshipwith Toxoptera aurantii in spatial-following relationships in the "WuNiuZao" tea garden in 2015. Although the three main enemies Tetragnatha maxillosa, Theridion octomaculatum Boes.et Str., and Tetragnatha maxillosa remained the same during the two years, the sorting was different in 2016. The relational grade analysis showed that the three main enemies Neoscona theisi, Theridion octomaculatum Boes. et Str., and Clubiona japonicola Boes. et Str had close relationship with Empoasca vitis in spatial-following relationships in the "WuNiuZao" tea garden in 2015. The three main enemies included Theridion octomaculatum Boes. et Str, Neoscona theisi, and Tetragnatha syusmata in 2016. Theridion octomaculatum Boes. et Str. and Neoscona theisi remained the same during the two years, but the seating arrangement was different. The relational grade analysis showed that the three main enemies Neoscona theisi, Clubiona japonicola Boes. et Str, and Tetragnatha syusmata had consanguineous relations with Breuipalpus oboyats in spatial-following relationships in the "WuNiuZao" tea garden in 2015. The three main enemies included Tetragnatha squamata Karsch, Clubiona japonicola Boes. et Str, and Theridion octomaculatum Boes et Str. Clubiona japonicola Boes. et Str and Tetragnatha squamata Karsch remained the same during the two years, but the sorting was different. The relational grade analysis showed that the three main enemies, Theridion octomaculatum Boes. et Str, Clubiona japonicola Boes. et Str, and Tetragnatha syusmata had consanguineous relations with Monolepta hieroglyphica in spatial-following relationships in the tea garden in 2015. The three main enemies included Tetragnatha syusmata, Clubiona japonicola Boes. et Str, and Neoscona theisi in 2016. Tetragnatha squamata Karsch and Clubiona japonicola Boes. et Str remained the same during the two years, but the sorting was different. The main reason for the change of the natural enemies of the pests during the two years is the difference in the ratio of the number of individual pests to the number of individual natural enemies:smaller the ratio, closer is the following relationship between predators and pests, which seems to be caused by a shortage in natural enemy species resources.