To investigate the mechanism of influence of different drying-rewetting frequencies on available soil nutrients and DOC,in this study, we collected cinnamon soil at a depth of 0-10cm below the surface in Beijing and manipulated soil water stress in the laboratory by exposing soil to 0, 1, 2, 3, or 4 drying-rewetting cycles for 58 days, including a 10 day pre-incubation period and a 48 day treatment period. After 58 days, principal soil fertility indexes such as available nutrients and DOC were tested to observe changes as a result of different drying-rewetting frequencies. Data were analyzed using single-factor variance analysis and the Least Significant Difference method (LSD) for the dependent groups. Available K and nitrate nitrogen were lower in the drying and rewetting treatments than in the constantly moist treatment(CM). Low drying-rewetting frequency might lead to the loss of available K, while K release might appear in the late stages of high drying-rewetting frequency conditions. As drying-rewetting frequency increased, the available K also increased. Four drying-rewetting cycles were critical for K release. Increasing the drying-rewetting frequency would result in frequent perturbations to the balance between various forms of nitrogen, and the mineralization rate would increase at the lowest drying-wetting frequency (DW1, 1 drying-rewetting cycle) and the highest drying-wetting frequency (DW4, 4 drying-rewetting cycles). As drying-wetting frequency increased, nitrate nitrogen of drying-rewetting treatment become closer to that of constantly moist (CM). Soil nitrate nitrogen significantly increased, while available K, ammonium nitrogen, and DOC significantly decreased, with drying-wetting frequency, and available P and pH were relatively insensitive to drying-rewetting frequency. In 48 days, with increase in drying-rewetting frequency (from 1 cycle to 2, 3, and 4 cycles) and decrease in drying-rewetting period (from 48d to 24, 16, and 12d) and the last period of each drying or wetting period (from 24d to 12, 8, and 6d), nitrate nitrogen and available K tended to increase to levels close to those of the constantly moist condition.