According to the requirements of "Research on Typical Fragile Ecological Restoration and Protection" in 2016, this project will focus on the study of integration and demonstration of key technology for eco-security guarantee in the urban agglomerations of Yangtze River Delta. The program aims to reveal the conjugation mechanism of multiple ecological security problems of urban agglomerations in Yangtze River Delta by the framework of DPSIR, and develop the collaborative interaction regulations and guarantee paths. Accordingly, one basic theory framework, five major key technologies of guaranteeing ecological security and one collaborative decision support system and platform are intended to be proposed and developed. Our achievements would improve the ability of ecosystems monitoring and management in Yangtze River Delta, promote regional healthy development, and provide significant references for the sustainable development of national urban agglomerations. It would provide significant social, economic and ecological benefits. The whole program is sponsored by the National Key Research and Development Program of China (No.2016YFC0502700).