An undisturbed sediment column sample covered by Shichito matgrass (Cyperus malaccensis) in the Shanyutan and Daoqingzhou wetland of the Min River estuary was selected to research the effects of salinity on the denitrification process of the wetland sediment in July 2015. Using the sealed anaerobic culture method, the denitrification rates, which varied with the culture time in different salinity conditions, were calculated. At the initial stage, from low salinity to high salinity culture conditions, the denitrification rates of the sediments in the Shanyutan wetland were (15.5±1.38), (4.28±8.46), and (12.94±0.24) mg kg^-1 d^-1, respectively. The values in the Daoqingzhou wetland, however, were (31.93±4.89), (30.66±5.375) and (36.69±3.44) mg kg^-1 d^-1, respectively. The decreased amplitudes of the denitrification rates after 10 days in 0 and 10 salinity conditions were 36.97%, and 53.01%, respectively, of the amplitudes after 5 days. The values were higher compared with 22.64% and 28.84% in the Daoqingzhou wetland. In the late culture stage, the denitrification rates showed decreasing trends down to zero. The denitrification activities in the Shanyutan and Daoqingzhou wetlands both showed trends of increasing. The variation ranges in the Shanyutan and and Daoqingzhou wetland were 95.6%-99.8% and 90.0%-96.7%, respectively. During the entire training period, the denitrifying activity in the Shanyutan wetland was higher than that in the Daoqingzhou wetland. The results reflected that the salinity had an inhibition effect on the denitrification rate of the sediment in the Min River estuary wetland.