In recent years, iron-rich groundwater from farmland drainage and upstream runoff has emerged as a serious environmental problem affecting into wetland ecosystems. This problem is further complicated by the background of climate change. A microcosm experiment was designed to investigate the responses of (Glyceria spiculosa (Fr.Schmidt.) Rosh.) to covarying iron (Fe) concentrations, hydroperiods, and temperatures, in order to better predict the future distribution of G.spiculosa in the Xiaoxingkai Lake wetlands. To test the entire growth cycle of G.spiculosa under conditions that prevail in the field, this study used three artificial climate chambers and a factorial design consisting of three Fe levels, three temperature settings, and three water levels. Plant response variables were height, leaf number, stem diameter, leaf area, chlorophyll a and b, biomass; also measured were total concentrations of iron, carbon, phosphorus, and nitrogen. The results showed the following: 1) The main effect of temperature on plant height, leaf number, and leaf area was significant, with the greatest values occurring in the -2℃ treatment. The temperature effect on chlorophyll a and b was significant, as well, with the greatest values in the ambient temperature treatment, likely because of the relatively slow growth rate and higher chlorophyll accumulation in cells; 2) The main effect of water level on stem diameter and total iron concentration of aboveground biomass was highly significant (P<0.001). The 5 cm waterlogging treatment was the most favorable for increasing Fe uptake and accumulation in plants, such that total Fe concentration aboveground was increased by 227.1% and 475.9% versus the 0 cm and -5 cm treatment levels, respectively. Lastly, 3) the main effect of Fe level on all the ecophysiological traits investigated were insignificant (P<0.05). Based on this comprehensive analysis of G.spiculosa in its different growing stages, we conclude that an increase in temperature had adverse effects on G.spiculosa growth, and that it performed the best in the -2℃ treatment. It is also noteworthy that G.spiculosa grew better under flooded conditions. However, the current Fe input concentrations should not significantly affect the growth of G.spiculosa. Presumably, under accelerated global warming, G.spiculosa-which was clearly sensitive to climate change-will face a near-certain threat. This could have consequences for the ecological structure and functions of these wetlands