In the present study, a spatial diffusion model of poisonous weed invasion by using inter-species competition model was developed based on poisonous weeds and edible forage in native pastures. Using the cellular automata theory, the competition model was extended to the spatial network to simulate and study the spatial distribution patterns of poisonous weeds, which will provide data supporting their control. The results showed that (1) under the influence of invasion and diffusion, the coexistence equilibrium point of the poisonous weeds and the edible grass could be increased by an increase of two, which increased the possibility of coexistence; and (2) the spatial distribution of the weed population was affected by invasion and diffusion, and the aggregation of the spatial distribution of the population was reduced.