Arbuscular mycorrhizal (AM) fungal colonization in the roots of 17 common plant species were measured to analysis the phylogenetic conservatism of AM colonization that are believed to be plant functional traits. The results showed that there were no phylogenetic signals of AM colonization, and its extant significant variation was explained more by relatively recent than ancient divergences in the phylogeny, suggesting that closely related plant lineages had no similar traits. Our study systematically reports the relationships between plant phylogeny and AM colonization in an alpine meadow ecosystem, and highlights no significant relevance between plant phylogeny and AM colonization.