Ecological redlines are the most important boundaries in the ecological protection zones, and the baseline of the ecological security of a nation or a region. The delineation of the redlines embodies the policy guidance to implement ecological protection forcibly and strictly. Based on the status quo of land use, we evaluated the ecosystem services of the ecological redlines of Jiangsu Province, which were delineated in 2013. The results showed that: (1) The ecosystem service value of the ecological redlines was 5.64×10¹⁰ RMB/a, accounting for 48.69% of the ecosystem service value of the entire land area of Jiangsu Province. (2) Water was the most important type of land use in Jiangsu Province. The ecosystem services value of water in the ecological redlines accounted for 78.34% of the ecosystem services values of all ecological redlines. (3) The per unit area value of ecosystem services of ecological redlines was 2.47×10⁴ RMB/hm², which was 2.18-fold that of the entire land area. The per unit area value of ecosystem services of wetland was the highest, and it was 4.89-fold that of the entire land area. Water was followed by forestland, and grassland in descending order. (4) In the order of magnitude, the ecosystems that accounted for its area in the entire province were wetlands, waters, forest, and grassland. The results were in accordance with the per unit area value of ecosystem services. This indicates that the greater the per unit area value of ecosystem services, the higher the level of protection achieved.