For the urban ecological protection and special planning, it is meaningful to analyze and identify urban ecological land, establish ecological security pattern and propose the strategy for developing construction land. As a case study of Anqing City, we divide ecological lands into three types, biological diversification protection, water resource security and geological disaster avoidance by the high resolution remote sensing imagery, and classify ecological land into three levers (very important, medium important and commonly important) by GIS spatial analysis method. The ecological protection area is 394.1km², accounting for land use planning area proportion of 44.5%, it is exploitation-prohibited region, and where can only build the necessary road and municipal utilities, ecological agricultural facilities, parkland and tour necessary facilities. Then we take the very important ecological land and the prohibited-construction areas stipulated by relevant laws, standards and policies as the source to gain Anqing City comprehensive ecological security pattern at different safety levels by using minimum cumulative resistance model. Beside the ‘Source’ and low security pattern of land, the city construction land will be 303.1km², and it is enough to the demand of land in urban master planning at 2030. At last, based on the ecological security pattern and ecological influence and construction potency of construction land, we divide the construction land into prioritized, moderate, restricted and prohibited zones, and then point out developing strategies for each zones.