In April 2014, we investigated the differences of spatial distribution of Spartina alterniflora invasion sequence on soil carbon and nitrogen in a Cyperus malaccensis marsh. We analyzed a non-invaded C. malaccensis marsh (A), S. alterniflora-invaded patch edge (B), and S. alterniflora-invaded marsh (C) of the Min River estuary by using new-generation space-time methods. The results showed that invasion of S. alterniflora significantly reduced the NO₃^--N content(P > 0.05) but increased the NH₄⁺-N content, which is mainly due to the invasion significantly changed the marsh soil particle composition (the sand content significantly increased by 33.81%) and promoted soil mineralization and nitrification, and vertical nitrate leaching. S. alterniflora invasion increased the carbon and nitrogen content and C/N ratio of soil. Comparing the C. malaccensis community and the invasive plate edge, soil carbon stock increased by 8.73% and 24.37%, respectively, while nitrogen stock increased by 10.22% and 17.87%, respectively, because the invasion of S. alterniflora resulted in a significant change of soil particle composition from marsh plant community structure, nutrient cycling and high siltation. With respect to the invasion of S. alterniflora in Yancheng (Jiangsu Province), Yangtze estuary, and Hangzhou Bay wetlands, the carbon and nitrogen contents of soil showed more significant changes by the invasion of S. alterniflora on C. malaccensis in the Min River estuary, and the S. alterniflora invasion could change the sources of terrestrial and marine organic matter in the soil, autogenously enhancing soil nutrients in marshes,too.