We used spatial analysis and other statistical methods with remote sensing image data and GIS technology to analyze the spatial distribution characteristics, and factors that influence this distribution, of isolated wetlands in the Sanjiang Plain, China. The mean center of the isolated wetlands is in the southwest, the long axis of the area is northeast-southwest, and the perimeter-to-area ratio of the ellipse is greater than that of the whole wetland (marsh, riverine wetland, and lake wetland). There are three "hot spots" of wetlands-in the central, northwest, and southwest of the Sanjiang Plain-which are influenced by human disturbance. Under natural conditions, isolated wetlands range in altitude 51-54 m, grade 1-3 degrees, distance to a river 12-16 km and the site of river terraces reach maximum height. Influenced by human factors, isolated wetlands are mainly distributed in residential areas, roads, and protected areas, and the relationship between the isolated wetlands and the drainage ditches is increased with distance. The number of isolated wetlands increased and then decreased under human activity. The spatial distribution of isolated wetlands in the Sanjiang Plain is a result of both natural and anthropogenic factors, and the protection of the isolated wetlands should be strengthened.